Functional tuning of a salvaged green fluorescent protein variant with a new sequence space by directed evolution... - ...
fluorescent protein (GFP) as a model protein. Here, in an attempt to mimic a step in the natural
evolution process of
proteins ... on both
proteins. The specific
mutation, K8N ...
The Molecular Structure of Green Fluorescent Protein - ... acid substitutions.
Mutation of Tyr66 in the fluorophore ... GFP expression19. Molecular
evolution techniques have been ... development of
fluorescent proteins with additional emission ...
Improved monomeric red, orange and yellow fluorescent proteins derived from Discosoma sp. red fluorescent protein - ... - ... Many new
fluorescent proteins with different colors have ... Notes online), the I197E
mutation may contribute a hydrogen ... be quite valuable.
Evolution of such
proteins or recombination of ...
Immune system contributes to evolution of a new fluorescent protein - ... contributes to
evolution of a new
fluorescent protein ... enormously faster
mutation of genes to produce
proteins with useful ... help researchers use
fluorescent proteins in intact mammals ...
The Whitney Laboratory for Marine Biology - ... quantify the effects of each
mutation involved in transition between the ... In press)
Evolution of function and color in GFP-like
proteins. In: Green
Fluorescent Protein: Properties ...
Immune system contributes to evolution of a new fluorescent protein - ... type of
fluorescent protein. The
mutation process, called somatic ... help researchers use
fluorescent proteins in intact mammals, because ... useful in directed protein
evolution. " It will ...
Immune system contributes to evolution of a new fluorescent protein - ... new type of
fluorescent protein. The
mutation process, called somatic ... in directed protein
evolution. " It will not be limited to the particular class of
proteins that we studied ...
HHMI News: Rapid Evolution of a New Fluorescent Protein - ... Rapid
Evolution of a New
Fluorescent Protein ... enormously faster
mutation of genes to produce
proteins with useful ... help researchers use
fluorescent proteins in intact mammals, because ...
Nikon MicroscopyU: Introduction to Fluorescent Proteins -
Fluorescent proteins provide the ability to visualize, track, and quantify molecules and events in living cells with high spatial and temporal resolution, essential features for understanding biology systems. ... With the rapid
evolution of
fluorescent protein technology, the utility of this ... This
mutation is present in the most popular varieties of
fluorescent proteins derived from Aequorea ...
Table of contents for Green fluorescent protein - Table of Contents for Green
fluorescent protein : properties, applications, and protocols / edited by Martin Chalfie and Steven R. Kain, available from the Library of Congress. ... Morin 3.
Evolution of Function and Color ...
Mutation of Green
Fluorescent Protein David A. Zacharias, Douglas Prasher, Roger Y. Tsien 7. Discovery and Properties of GFP-like
Proteins ...